. Author manuscript; available in PMC: 2015 Dec 1.

Published in final edited form as: Biochem Biophys Res Commun. 2013 Oct 8;440(4):743–748. doi: 10.1016/j.bbrc.2013.09.137

TABLE 1.

Parameters of standard phospholipids analyzed by direct infusion ESI/MS methods. The parameters of the internal mixture corresponding to each class of phospholipid species are shown. All ions were monitored in the positive ion mode. PC – phosphatidylcholine; PI – phosphatidylinositol; PE p phosphatidylethanolamine; PS – phosphatidylserine; PG – phosphatidylglycerol; PA phosphatidic acid; PL – phospholipid; IS – internal standard; HG – head group; Prec – precursor scan; NL – neutral loss scan.

PL Class	Internal Standard	IS Mass	Adduct	IS Formula	HG Fragment	ESI-MS scan mode (HG fragment mass)
PC	PC 13/13	650.47	[M+H]+	C₃₄H₆₈O₈PN	phosphocholine	Prec (184)
PI	PI 16/16	828.52	[M+NH₄]+	C₄₁H₇₉O₁₃P	phosphoinositol	NL (277)
PE	PE 15/15	664.48	[M+H]+	C₃₅H₇₀O₈PN	phosphoethanolamine	NL (141)
PS	PS 12/12	624.50	[M+H]+	C₃₀H₅₈O₁₀PN	phosphoserine	NL(185)
PG	PG 12/12	628.38	[M+NH₄]+	C₃₀H₅₉O₁₀P	phosphoglycerol	NL (189)
PA	PA 16/18:1	692.49	[M+Nh₄]+	C₃₇H₇₁O₈P	phosphate	NL (115)