Table 2.
Plant biomass-degrading enzymes found in the RWS metasecretome
| Protein number | CAZy family | Action on (activity)a | Best PSI-BLAST hit vs. NCBI (organism source) | Accession no. | COV (%) | e value | Identity (%) | COV2 | #P |
|---|---|---|---|---|---|---|---|---|---|
| RWS1 | CBM48|GH13|CBM41 | Alpha-glucan polysaccharides (alpha-amylase/pullulanase) | Pullulanase (Klebsiella) | EHS90708.1 | 100 | 3E−45 | 98 | 43 | 5 |
| RWS2 | CBM48|GH13|CBM41 | Pullulanase (Klebsiella) | WP_017144955.1 | 100 | 8E−48 | 100 | 37 | 3 | |
| RWS3 | CBM48|GH13|CBM41 | Pullulanase (Klebsiella) | EHS90708.1 | 100 | 9E−34 | 97 | 33 | 3 | |
| RWS4 | CBM48|GH13|CBM41 | Pullulanase (Klebsiella) | EHS90708.1 | 100 | 4E−41 | 96 | 28 | 3 | |
| RWS5 | CBM48|GH13|CBM41 | Pullulanase (Klebsiella) | EHS90708.1 | 100 | 2E−64 | 99 | 45 | 3 | |
| RWS6 | CBM48|GH13|CBM41 | Pullulanase (Klebsiella) | EHS90707.1 | 100 | 1E−91 | 98 | 34 | 4 | |
| RWS7 | CBM48|GH13|CBM41 | Pullulanase (Klebsiella) | WP_017144955.1 | 100 | 2E−41 | 97 | 48 | 5 | |
| RWS8 | CBM48|GH13|CBM41 | Pullulanase (Klebsiella) | EHS90708.1 | 100 | 2E−100 | 95 | 30 | 3 | |
| RWS9 | GH10 | Hemicellulose (endoxylanase) | XynB19 precursor (Sphingobacterium) | ACX30647.1 | 100 | 9E−47 | 95 | 57 | 5 |
| RWS10 | GH10 | Endo-1,4-beta-xylanase precursor (Sphingobacterium) | ACX30652.1 | 100 | 4E−67 | 95 | 38 | 3 | |
| RWS11 | GH10 | Endo-1,4-beta-xylanase precursor (Sphingobacterium) | ACX30652.1 | 98 | 1E−101 | 92 | 27 | 5 | |
| RWS12 | GH12 | Cellulose (endoglucanase) | Peptidase M16 (Sphingobacterium) | WP_045752104.1 | 100 | 3E−96 | 97 | 17 | 2 |
| RWS13 | GH16 | (Hemi)cellulose (endoglucanase/endogalactanase) | Glycerophosphodiester phosphodiesterase (Sphingobacterium) | WP_031286917.1 | 100 | 2E−70 | 99 | 55 | 5 |
| RWS14 | GH16 | Glycerophosphoryl diester phosphodiesterase (Sphingobacterium) | KKX48537.1 | 100 | 7E−50 | 100 | 34 | 3 | |
| RWS15 | GH16 | Glycerophosphoryl diester phosphodiesterase (Sphingobacterium) | KKX48537.1 | 100 | 5E−77 | 100 | 24 | 3 | |
| RWS16 | GH16 | Glycerophosphodiester phosphodiesterase (Sphingobacterium) | WP_031286917.1 | 100 | 2E−95 | 99 | 41 | 5 | |
| RWS17 | GH3 | Cellulose (beta-glucosidase) | Beta-glucosidase (Sphingobacterium) | CDS95530.1 | 100 | 7E−106 | 95 | 21 | 3 |
| RWS18 | GH3 | Glycoside hydrolase family 3 (Sphingobacterium) | KKX49060.1 | 81 | 1E−52 | 92 | 27 | 2 | |
| RWS19 | GH3 | Glycoside hydrolase family 3 (Sphingobacterium) | KKX49060.1 | 100 | 9E−94 | 92 | 21 | 3 | |
| RWS20 | GH3 | Glycoside hydrolase family 3 (Sphingobacterium) | KKX49060.1 | 75 | 3E−34 | 96 | 36 | 2 | |
| RWS21 | GH43 | Hemicellulose (beta-xylosidase/alpha-arabinofuranosidase) | Aldose epimerase (Sphingobacterium) | WP_045756400.1 | 100 | 4E−81 | 81 | 11 | 2 |
| RWS22 | GH43 | GH43D19 precursor (Sphingobacterium) | ACX30655.1 | 100 | 3E−83 | 98 | 29 | 3 | |
| RWS23 | GH43 | GH43D19 precursor (Sphingobacterium) | ACX30655.1 | 100 | 1E−51 | 100 | 37 | 2 | |
| RWS24 | GH43 | GH43D19 precursor (Sphingobacterium) | ACX30655.1 | 97 | 8E−40 | 94 | 38 | 2 | |
| RWS25 | GH43 | GH43A19 precursor (Sphingobacterium) | ACX30649.1 | 100 | 3E−88 | 98 | 15 | 2 | |
| RWS26 | GH51 | Hemicellulose (alpha-arabinofuranosidase) | Alpha-l-arabinofuranosidase (Sphingobacterium) | WP_045756399.1 | 100 | 1E−88 | 99 | 27 | 2 |
| RWS27 | GH51 | Alpha-l-arabinofuranosidase (Sphingobacterium) | WP_045756399.1 | 92 | 5E−45 | 99 | 44 | 2 | |
| RWS28 | GH51 | Alpha-l-arabinofuranosidase (Sphingobacterium) | WP_038697950.1 | 100 | 3E−69 | 96 | 33 | 2 | |
| RWS29 | GH51 | Alpha-l-arabinofuranosidase (Sphingobacterium) | WP_045756399.1 | 100 | 3E−52 | 100 | 43 | 2 | |
| RWS30 | GH67 | Hemicellulose (alpha-glucuronidase) | Alpha-glucuronidase (Sphingobacterium) | WP_031288024.1 | 90 | 5E−69 | 83 | 15 | 2 |
| RWS31 | GH95 | Hemicellulose (alpha-fucosidase) | Alpha-l-fucosidase (Sphingobacterium) | WP_045753034.1 | 99 | 8E−72 | 76 | 37 | 4 |
| RWS32 | GH95 | Alpha-l-fucosidase (Sphingobacterium) | WP_045753034.1 | 100 | 1E−44 | 82 | 46 | 3 | |
| RWS33 | GH95 | Alpha-l-fucosidase (Sphingobacterium) | WP_045753034.1 | 100 | 5E−71 | 77 | 37 | 4 | |
| RWS34 | AA5 | Lignin or polysaccharides (glyoxal/galactose oxidases)b | Cell envelope biogenesis protein OmpA (Flavobacterium) | WP_042565523.1 | 100 | 2E−44 | 99 | 18 | 2 |
| RWS35 | AA5 | Outer membrane protein omp38 (Acinetobacter) | WP_004819145.1 | 66 | 7E−53 | 98 | 39 | 5 | |
| RWS36 | AA5 | Flagellar motor protein MotB (Sphingobacterium) | WP_031288562.1 | 100 | 4E−82 | 98 | 24 | 4 | |
| RWS37 | AA5 | Flagellar motor protein MotB (Sphingobacterium) | WP_038696344.1 | 98 | 4E−53 | 97 | 65 | 6 | |
| RWS38 | AA5 | Membrane protein (Pseudomonas) | WP_016485176.1 | 100 | 8E−81 | 100 | 29 | 3 |