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. 2015 Dec 1;16:144. doi: 10.1186/s12931-015-0302-7

Fig. 5.

Fig. 5

Immunostaining of TNF receptor I expression of lung fibroblasts in 2D and 3D culture systems. TNF-α receptor I on fibroblast surface was immunostained with Alexa Fluor® 488 secondary antibody (green) in both 2D (ab) and 3D (cd) culture systems and nuclei were labeled with DAPI (blue). (Bar =50 μm, n = 3)