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Effects of glucose on the initial reaction rates of Bgl6 and the mutants. The reactions were performed at 50 °C and pH 6.0 with p-Nitrophenyl-β-d-glucopyranoside (pNPG) as substrate. The IC 50 values are 3.5 M (Bgl6), 0.3 M (V174A), 2.5 M (V174C), 2.5 M (A404 V), 3.0 M (L441F), and 3.0 (M3). Data represent the means of three experiments and error bars represent standard deviation
