Fig. 5.

Hydrolysis of cellobiose and pretreated SCB by Bgl6 and mutant M3. The reactions were performed at 50 °C in 100 mM phosphate buffer (pH 6.0). The concentrations of the substrates were 10 % (w/v). a Hydrolysis of cellobiose by Bgl6 (filled square) and mutant M3 (filled circle). b Celluclast 1.5 L (filled diamond) was used alone as a control. Addition of Bgl6 (filled square) and mutant M3 (filled circle) to Celluclast 1.5 L improved the SCB conversion. Data represent the means of three experiments and error bars represent standard deviation