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. 2015 Dec 1;8:79. doi: 10.1186/s13041-015-0172-4

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© Shimojo et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Derivation of motor neurons from hiPSCs. a Immunocytochemical analysis of motor neurons derived from 201B7, TIGE-9 and YFE-16 for HB9, ISL-1, and βIII-Tubulin after 2 weeks of monolayer differentiation and ChAT after 4 weeks of monolayer differentiation. Scale bar, 100 μm. b Quantitative analysis of the number of the cells positive for motor neuron markers in cultures derived from each hiPSC clone. n = 3, mean ± SEM. c Time-course analysis of the expression of HB9, ISL-1, and ChAT during monolayer differentiation of hiPSC-derived motor neurons via quantitative RT-PCR. n = 3, mean ± SEM. d Western blot analysis of the expression of the HB9, ISL-1, and ChAT proteins during monolayer differentiation of hiPSC-derived motor neurons. B7, 201B7. T9, TIGE-9. Y16, YFE-16. e Quantitative analysis of the expression of the HB9, ISL-1, and ChAT proteins through densitometry using ImageJ. Protein expression levels are normalized to β-Actin. n = 3, mean ± SEM. *, p < 0.05, (Student’s t test)