Table 1:
Antibodies used in this study.
| Antibody | Source | Dilution for GFP-Trap purified protein | Dilution for whole-cell lysate |
|---|---|---|---|
| Actin | Sigma-Aldrich (A 3853) | 1:1000 | |
| APC3 | Abcam (ab10538) | 1:1000 | |
| Aurora A | BD Biosciences (610939) | 1:1000 | |
| Biotin | Cell Signaling (7075) | 1:100 | 1:1000 |
| Cdh1 | Tim Hunt (Clare Hall Laboratories, London Research Institute, South Mimms, United Kingdom) | 1:50 | |
| FK2 | Enzo (PW0150) | 1:600 | 1:2000 |
| GFP | Abcam (ab290) | 1:2000 | |
| GFP | Roche (11814460001) | 1:500 | |
| K11 | Millipore (MABS107-I) | 1:100–1:500 | 1:1000 |
| K48 | Abcam (ab140601) | 1:500 | |
| Phospho–Histone H3 | Cell Signaling | 1:1000 | |
| Tubulin | Abcam | 1:2000 | |
| UBE2S | Abnova (PAB1701) | 1:1000 |