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. 2015 Dec 1;26(24):4333–4346. doi: 10.1091/mbc.E15-06-0422

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© 2015 Gong, Renigunta, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Freeze-fracture images of claudin-transfected LLC-PK1 cells. Freeze-fracture electron microscopy revealed TJ ultrastructures in LLC-PK1 cells with no transfection (A) or LLC-PK1 cells transfected with claudin-16 (B), claudin-19 (C), claudin-16 and -19 (D), claudin-16-246A mutant with claudin-19 (E), or claudin-16 with claudin-19-178A mutant (F). Bar, 100 nm. Quantitative image analyses revealed TJ strand difference (G) and TJ complexity difference (H) among these LLC-PK1 cells transfected with claudin-16, -19, or their mutants. *p < 0.05, n = 7–9.