FIGURE 10:

Oligomerization-induced trafficking is not associated with UPR activation. (A) Immunoblots using anti-Bip or anti-tubulin antibodies of extracts from nontransfected cells (lanes 1–3) or cells transfected with GPP130-GFP or GPP130-FM (lanes 4–6). The cells were untreated (Ø), tunicamycin treated (Tun) to induce the UPR, Mn treated (Mn) to cause endogenous GPP130 redistribution, left in AP (AP), or subjected to AP washout (w/o) to cause oligomerization of GPP130-FM. (B) Quantification of Bip recovery as a percentage of Bip levels in untreated cells (mean ± SEM, n = 3).