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. 2015 Dec 1;26(24):4427–4437. doi: 10.1091/mbc.E15-04-0207

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© 2015 Tewari et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — GGA1-DN blocks GPP130-FM trafficking. (A) Cells were cotransfected with HA-tagged GPP130-FM and either GFP-tagged GGA1 or GGA1-DN and then either left in AP or treated with a 1 h of AP washout. Images show GFP fluorescence from the transfected proteins and anti-HA staining, with the final panel using uniform thresholding of the GPP130-FM image to aid visualization of small punctae. Bar, 5 μm. (B) Quantified appearance of GPP130 in peripheral punctae of cells expressing GGA1 or GGA1-DN after 0 or 1 h of AP washout (mean ± SEM, n = 3, >10 cells/experiment, *p < 0.05).