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. 2015 Dec 1;26(24):4427–4437. doi: 10.1091/mbc.E15-04-0207

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© 2015 Tewari et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Clathrin inhibitor blocks oligomer-induced GPP130 trafficking. (A) GPP130-FM– transfected cells in the presence of AP were washed into serum-free medium lacking AP and containing either vehicle alone (DMSO) or vehicle containing the clathrin inhibitor Pitstop2. The cells were fixed and analyzed after 10 min to minimize indirect and toxic effects of Pitstop2. Images show GFP fluorescence from GPP130-FM and anti-giantin staining to mark the Golgi, as well as thresholded GPP130 images to mark cytoplasmic punctae. Note that Pitstop2 blocked GPP13-FM appearance in punctae. Bar, 5 μm. (B) Quantified appearance of GPP130-FM in peripheral punctae of control and Pitstop2-treated cells after AP washout (mean ± SEM, n = 3, >10 cells/experiment, *p < 0.05).