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. 2015 Dec 1;26(24):4427–4437. doi: 10.1091/mbc.E15-04-0207

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© 2015 Tewari et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — GGA1-DN blocks Man1-FM trafficking. (A) Images show GFP fluorescence of GGA1 or GGA1-DN and anti-HA staining of Man1-FM after 1 h of AP washout. Thresholded Man1-FM staining is also shown. Bar, 5 μm. (B) Quantified number of cytoplasmic punctae per cell for Man1-FM at 0 or 1 h after AP washout (mean ± SEM, n = 3, >10 cells/experiment, *p < 0.05). (C) Cells were cotransfected with HA-tagged Man1-FM and GFP-tagged Rab7 and then treated with a 1-h AP washout. Images compare GFP fluorescence and anti-HA staining with arrowheads marking examples of costained punctae. Bar, 10 μm. (D) The percentage of total Man1-FM punctae counted that were positive for Rab7 is quantified for the indicated time points (mean ± SEM, n = 3, >10 cells/experiment, p < 0.05 for 0 h vs 1 or 5 h).