Fig. 6.

Osteogenic response of BM-MSCs to BMP-2 after culture in SFM on TCP, BM-ECM, and CELLstart™. Early passage (P1) cultures of BM-MSCs were cultured on TCP, BM-ECM, and CELLstart™ in SFM for 7 days; the media were then changed to α-MEM containing 2 % FBS, and the incubation continued for an additional 24 hours before treatment with BMP-2 (60 ng/ml) for 48 hours. Expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and Runx2 was measured by TaqMan polymerase chain reaction. The mean ± standard deviation was calculated on the basis of three independent experiments. *P < 0.05 versus untreated. α-MEM alpha-minimum essential medium, BM-ECM bone marrow-derived extracellular matrix, BM-MSC bone marrow-derived mesenchymal stem cell, BMP-2 bone morphogenetic protein-2, FBS fetal bovine serum, GAPDH glyceraldehyde-3-phosphate dehydrogenase, P1 passage 1, Runx2 Runt-related transcription factor-2, SFM serum-free media, TCP tissue culture plastic