Table 1.

Percent positive cells obtained from cultures on the various substrates

		SSEA-4	CD73	CD90	CD105	CD146
Time in culture	Culture substrate
4 days	TCP	29 ± 4	45 ± 4	45 ± 5	50 ± 4	24 ± 4
	CELLstart™	46 ± 5	50 ± 7	49 ± 4	47 ± 5	59 ± 7
	BM-ECM	64 ± 6	67 ± 7	67 ± 5	65 ± 6	68 ± 7
7 days	TCP	N/A	N/A	N/A	N/A	N/A
	CELLstart™	45 ± 7	44 ± 8	61 ± 8	46 ± 9	65 ± 8
	BM-ECM	81 ± 10	80 ± 6	81 ± 7	70 ± 6	83 ± 9

TCP tissue culture plastic, BM-ECM bone marrow-derived extracellular matrix, N/A not applicable

Early passage (P1) cultures of BM-MSCs were grown on TCP, BM-ECM, or CELLstart™ in SFM for 4 and 7 days. Phenotypic expression of MSC-associated markers (SSEA-4, CD73, CD90, CD105, and CD146) was assessed by using flow cytometry. The data are the percent positive cells of the total cell population expressing each marker. The experiment was performed in triplicate, and the mean ± standard deviation is shown. Each experiment was performed three times