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. 2015 Dec 1;15:109. doi: 10.1186/s12896-015-0223-z

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© Stadlmann et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Schematic view of scFv fragment cloned into the expression vector pET28a (+). V_H and V_L fragments assemble to full-length scFv in between the linker peptide and are ligated into pET28a (+) vector, which provides a C-terminal hexahistidine tag