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. 2015 Dec 1;15:109. doi: 10.1186/s12896-015-0223-z

Fig. 3.

Fig. 3

Schematic view of scFv fragment cloned into the expression vector pET28a (+). VH and VL fragments assemble to full-length scFv in between the linker peptide and are ligated into pET28a (+) vector, which provides a C-terminal hexahistidine tag