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. 2015 Dec 1;15:109. doi: 10.1186/s12896-015-0223-z

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© Stadlmann et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — ELISA based testing of scFv candidates for reactivity to PT-Gliadin. Bacterial crude extracts expressing candidate scFvs were tested for antigen binding by ELISA as described in Methods. Samples were diluted 10-fold in TBS supplemented with 0.05 % Tween and 1 % BSA. Mock represents the negative control (crude extracts of bacteria transformed with empty vector). Data shown are OD (450 nm) levels and represent mean values (+/− SD) of duplicates