Fig. 8.

IgY competes with scFv for antigen binding sites. a. IgY competitively displaces scFv from PT-Gliadin coated plates. Competitive ELISA was performed with a 60 min pre-incubation with anti-PT-Gliadin IgY at indicated concentrations followed by addition of anti-PT-Gliadin-scFv at a concentration of ~ 0.04 [μg/ml] for 40 min. Detection was performed with anti penta-His antibody, as it detects specifically the His-tag labelled scFv but does not react with IgY. ELISA principle is described in Methods. Data shown are OD (450 nm) values and represent mean values (+/−SEM) of triplicates. b. IgY competitively displaces scFv from wheat flour digest coated plates. Competitive ELISA was performed as described in A and Methods. Data shown are OD (450 nm) values and represent mean values (+/−SEM) of triplicates