Table 1.

Equilibrium binding constants of scFv for PT-Gliadin and wheat digests

Antigen	Kaff mean (+/− SEM) [M−1]
PT-Gliadin	3.82 (+/− 1.93) x 107
Wheat flour digest	3.21 (+/− 2.14) x 107
Kamut flour digest	2.30 (+/− 1.49) x 107

Data were analyzed by 3-parameter curve-fit nonlinear regression (Graph Pad Prism 6) as described in Methods. Mean affinity constants (+/− SEM) are shown