Fig. 2.
Overlaid chromatogram of the marker peptides included in the multiplexed targeted proteomic assay. The assay was developed to quantitate 74 peptides in a 10 min LC run. Markers significant in the study are shown in the above overlaid chromatogram except for transferrin, serum amyloid A4 and apolipoprotein E which are not shown due to interference from other peaks. All markers are shown individually as endogenous and spiked in Additional file 8: Figure S2