Table 1.

Determination of the coating concentration of protein and serum dilution in optimization of the indirect ELISA method

Antisera at different dilutions	OD450 values of antigen at different coating concentrations
	0.06 μg	0.12 μg	0.24 μg	0.48 μg	0.96 μg	1.92 μg
1:5 (P)	1.181	1.137	1.065	1.078	1.182	1.178
1:5 (N)	0.427	0.361	0.319	0.307	0.478	0.487
1:10 (P)	0.932	0.908	0.896	0.789	0.858	0.873
1:10 (N)	0.357	0.288	0.246	0.238	0.357	0.371
1:20 (P)	0.664	0.621	0.542	0.501	0.645	0.626
1:20 (N)	0.238	0.244	0.191	0.180	0.275	0.245
1:40 (P)	0.461	0.447	0.347	0.397	0.473	0.445
1:40 (N)	0.198	0.165	0.131	0.148	0.223	0.227
1:80 (P)	0.348	0.339	0.298	0.305	0.392	0.381
1:80 (N)	0.157	0.143	0.115	0.122	0.134	0.139

Note: N, positive serum; P, negative serum; The values in bold represent optimum conditions of this indirect ELISA method