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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Eur J Immunol. 2015 Jun 25;45(9):2582–2592. doi: 10.1002/eji.201445371

Figure 3. Tregs from term and preterm neonates suppress Tcon activation, DC maturation and actin polymerization in DC:Tcon aggregates.

Figure 3

Activated Tregs from peripheral blood from adults (n=8) and cord blood from preterm (n=6) and term (n=8) neonates were added or not to DC:Tcon cultures at a 1:10:1 (DC:Tcon:Treg) ratio and cultured for 24h. (A) Percentages of CD134+ Tcon, (B) CD83+ DC in aggregates and C) actin polymerization at the IS in presence or absence of Treg were determined by imaging flow cytometry. (A-C) Results are representative of five independent experiments performed. Each dot shows represent a single donor. Treg suppression was analyzed using Wilcoxon tests.