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. 2015 Dec;100(12):1517–1525. doi: 10.3324/haematol.2015.128736

Figure 4.

Figure 4.

Nociceptin receptor mediates the analgesic effect of AT-200. HbSS-BERK mice were injected with naloxone (1 mg/kg), a non-selective opioid receptor antagonist or SB-612111 (10 mg/kg), a selective NOP receptor antagonist, 10 min before injecting AT-200 (10 mg/kg) or were administered vehicle, AT-200 or naloxone alone. Measures of (A) PWF, (B) Grip Force, and (C) PWL to heat are shown. SB-612111 antagonized AT-200–induced anti-nociceptive effect, but naloxone did not. Data are shown as mean ± SEM from 5–7 HbSS-BERK mice per treatment. Statistical significance was calculated by comparing each value to BL values (*), between vehicle and treatment groups (†).*P<0.05 and **P<0.005 compared to BL; †P<0.05 and ††P<0.005 compared to vehicle for that time point. Mean age of mice ± SEM in months were, HbSS-BERK Naloxone (n=6), 6.2 ± 0.08; HbSS-BERK SB-612111 + AT-200 (n=7), 5.42 ± 0.06; and HbSS-BERK Naloxone + AT-200 (n=7), 5.5 ± 0.09.