Figure 1.

Distinct miRNA expression patterns in CD4⁺ and CD8⁺ T cells of AA patients. Volcano plots of results from 84 miRNA known to be involved in lymphocyte activation in (A) CD4⁺ and (C) CD8⁺ T cells (AA patients, n=12; healthy donors, n=8) and (E) CD19⁺ B cells (AA patients, n=9; healthy donors, n=7) using miRNA PCR-array. The x-axis is the estimated difference in expression measured in log₂; vertical lines refer to a 3-fold difference in expression between the two groups. MiRNA highly expressed in AA or healthy donors are on the right or the left, respectively. The y-axis is the significance of the difference measured in −log₁₀ of the P-value; the horizontal line represents our cutoff for significance at P<0.01. Hierarchical clustering of miRNA in (B) CD4⁺ T cells, (D) CD8⁺ T cells, or (F) CD19⁺ B cells was visualized by heatmap analysis. A red-blue color scale depicts normalized miRNA expression levels in Ct values (red: high, blue: low). (G) Venn diagram presentation of PCR array data.