Figure 4.

The cognate cas3 is required for Cst and Csa plasmid DNA silencing. Plasmid challenge assay results plotted for single or double cas3 gene deletion strains (A), Cst derivative strains (B), and Csa derivative strains (C). Colony forming units are plotted on the Y-axis, with error bars indicating the standard deviation in at least three replicates. Transformed plasmids contained no target (light gray bars) or the 7.01 target with GGG PAM (dark gray bars). Strain transformed and proteins(s) expressed from plasmids are indicated on the X-axis. Cases in which no colonies were observed are indicated as ‘bdl’ for below detection limit of the assay.