Figure 3.

Mitochondrial dRNaseZ is autonomously required for imaginal disc cell proliferation. (A,C) Fluorescent images of third instar eye discs containing RNZ^WT and RNZ^ΔMTS clones in a wild-type background. Yellow dots outline RNZ^WT and RNZ^ΔMTS clones (GFP negative); red dotes outline the wild-type twin-spots (double GFP). Nuclei are stained with Hoechst. (B,D) Areas of clones and corresponding twin-spots measured in pixels with histogram function of Adobe Photoshop. Bars are ordered on the X-axis according to the size of the twin-spot (blue, GFP+). Values for median and average clone areas are indicated. RNZ^WT clones (B) have similar sizes with wild-type twin-spots (P = 0.67), while RNZ^ΔMTS clones (D) are smaller (P < 0.0001, t-test). (E,F) BrdU incorporation (E) and PH3 staining (F) posterior of the morphogenetic furrow in third instar eye discs. In merge: GFP, green; BrdU or PH3, red; Hoechst, blue. GFP negative RNZ^WT and RNZ^ΔMTS clones are outlined with yellow dots, and discs are oriented with anterior to the right. Note that RNZ^ΔMTS clones have thinner BrdU band and no PH3 staining.