Table 4. Variables.

Variable	Section first used	Meaning
j	Perfect PCR	PCR cycles
N0	Perfect PCR	Initial copy number
n(j)	Perfect PCR	Number of molecules after j cycles
N0, x	PCR bias	Initial copy number of sequence x
nx(j)	PCR bias	Number of molecules with sequence x after j cycles
cx	PCR bias	PCR efficiency of sequence x
mx	PCR bias	Estimated PCR efficiency of sequence x
Pamp	Stochastic amplification	Probability of amplification per cycle
s0	Template switching	Per molecule probability of switching
sj	Template switching	Probability of template switching on cycle j
Sj	Template switching	Total number of template switched on cycle j
Qm	Template switching	Total number of template switched molecules after m cycles
Nk	Template switching	Total number of molecules after k cycles
Jk	Template switching	Approximation of from the data
c	Polymerase errors	PCR efficiency
Rj	Polymerase errors	Number of faithfully copied molecules after j cycles
e	Polymerase errors	Probability of making one error per molecule per cycle
Zk	Polymerase errors	Total number of molecules containing a single error after k cycles
Fk	Polymerase errors	Fraction of all sequences containing errors