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. 2015 Jul 14;43(21):e144. doi: 10.1093/nar/gkv718

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Scheme for imaging RNA by using contact-quenched fluorogenic probes. (A) The contact-quenched fluorophore-dinitroaniline conjugates (OFF) light up upon binding to the quencher binding aptamer (ON). RNA of interest can be fused to the quencher binding aptamer and imaged in the presence of the fluorophore-quencher conjugate. F denotes any fluorophore and Q denotes a contact quencher. (B) Structures of the contact quencher and fluorogenic probes used in this study. Dinitroaniline (DN) is the contact-quencher used in this work. FL-DN: fluorescein-dinitroaniline, RG-DN: rhodamine green dinitroaniline, TMR-DN: tetramethylrhodamine-dinitroaniline, SR-DN: sulforhodamine-dinitroaniline, TR-DN: TexasRed-dinitroaniline.