Figure 5.
Imaging DNB aptamer in live E. coli with various fluorophore-dinitroaniline conjugates. Bacteria were transformed with either pET28-DNB or pET28-tRNA plasmid. Transcription was induced with IPTG. Bacteria were incubated with 1 μM of RG-DN (green), TMR-DN (yellow) and SR-DN (red) for 5 min and imaged at 37°C. Scale bar, 5 μm.