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. 2015 Jul 14;43(21):e144. doi: 10.1093/nar/gkv718

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Dual colour labelling strategy using a quencher-binding aptamer and a fluorophore-binding aptamer. (A) Cloning vector used for expressing both SRB-2 and DNB (pET-SRB-2-DNB) aptamers in E. coli. (B) Structure of the two fluorogenic probes used for dual-colour imaging, SR-MN: sulforhodamine B-p-nitrobenzylamine and RG-DN: Rhodamine green-dinitroaniline. (C) Imaging DNB and SRB-2 aptamers in live E. coli with RG-DN and SR-MN (1 μM, each), respectively. Fluorescence signal in both red and green channel was detected in cells expressing both DNB and SRB-2, while cells expressing either DNB or SRB-2 showed fluorescence only in the green or red channel, respectively. Cells expressing the tRNA scaffold showed no fluorescence signal. Scale bar, 5 μm.