Table 1. Spectroscopic properties of free and DNB aptamer-bound probes measured in 1X selection buffer at 25°C.
| Probe | Ex (nm) | Em (nm) | Extinction coefficient (M−1cm−1) | KD (μM) | Quantum yield | Brightnessa |
|---|---|---|---|---|---|---|
| FL-DN/DNB | 498 | 528 | 51 750 | 8.55 ± 0.53 | NDb | ND |
| FL-DN | 496 | 523 | 63 806 | 0.051 | 15 | |
| RG-DN/DNB | 507 | 534 | 37 350 | 4.48 ± 0.60 | 0.32 | 55 |
| RG-DN | 502 | 529 | 42 849 | 0.074 | 15 | |
| TMR-DN/DNB | 555 | 582 | 47 150 | 0.35 ± 0.05 | 0.90 | 195 |
| TMR-DN | 554 | 582 | 57 206 | 0.080 | 21 | |
| SR-DN/DNB | 572 | 591 | 50 250 | 0.80 ± 0.10 | 0.98 | 226 |
| SR-DN | 568 | 589 | 46 314 | 0.027 | 6 | |
| TR-DN/DNB | 598 | 619 | 52 366 | 18.0 ± 1.8 | ND | ND |
| TR-DN | 591 | 611 | 45 600 | 0.086 | 18 | |
| GFP | 395 | 508 | 27 600 | - | 0.79 | 100 |
aBrightness (extinction coefficient × quantum yield) is reported relative to GFP.
bND stands for not determined.