Figure 1.

Comparison of MethylC-seq and 4mC-TAB-seq. (A) MethylC-seq converts C and a portion of 4mC to T. The remaining 4mC and almost all 5mC will be read as C. (B) 4mC-TAB-seq converts C, 5mC and a portion of 4mC to T, whereas about half of 4mC will be exclusively read as C. (c) Properties of 4mC and 5mC under different treatment conditions. Untreated or Tet-treated 4mC/5mC-containing model DNA is applied to either standard or optimized bisulfite treatment condition. Samples were PCR amplified, subcloned into TOPO vector and Sanger sequenced to quantify the number of 4mC or 5mC being read as C.