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. 2015 Aug 28;43(21):10213–10226. doi: 10.1093/nar/gkv842

Figure 4.

Figure 4.

Sir2 mutants are functional in rDNA silencing and recombination. (A) KRY360 was transformed with either vector or Sir2 wild type or Sir2 mutant plasmids along with SIZ2 and tested for growth on 5-FOA to measure silencing of rDNA locus. Wild type Sir2 and all point mutants of Sir2 restore silencing at the rDNA. (B) KRY 1541 was transformed with plasmid encoding either WT Sir2 or Sir2K215R or Sir2–SUMO and recombination frequencies were estimated. Only red/white half sectors were counted.