Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 28;43(21):10213–10226. doi: 10.1093/nar/gkv842

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 5. — Interaction of Sir2 with Sir4 and Net1. (A) Protein extracts from KRY868 containing myc-tagged Sir4 or KRY875 containing myc-tagged Net1 (B) with indicated plasmids were immunoprecipitated with anti-myc antibodies and immunoblotted with myc or Sir2 antibody to test co-immunoprecipitation of Sir2 with Sir4 and Net1. Whole cell extracts and flow though are shown for comparison. (C) Interaction of Sir4 with Sir2 in yeast 2-hybrid assay. Gbd fusion of Sir2, Sir2K215R and Sir2–SUMO were cotransformed with either empty GAD vector or GAD vector encoding SIR4 (C) or Net1 (D). Growth on SC-Leu Ura indicates that both plasmids are present. Growth on SC-His and SC-Ade indicates interaction between the Gbd and Gad fusion plasmids.