Skip to main content
. 2015 Oct 3;43(21):10534–10545. doi: 10.1093/nar/gkv996

Figure 5.

Figure 5.

Characterization of solution behavior of hSerRS. (A) The chromatograms of free tRNA, the E447K mutant and complexes formed at different molar ratios on a Superdex 200 column (10/30). The species eluted from the column are represented by the solid circles, while the orange circles stand for tRNA molecule and the dark cyan V-shaped symbol for the hSerRS dimer. The retention volumes of each eluted species (indicated by the arrows) are given in the top right corner. (B) EMSA of hSerRS-tRNA complexes with a constant amount of protein (167 pmol) and increasing amounts of tRNA (molar ratios of protein to RNA ranging from 2:0.3 to 2:4.8). The two identical sets of samples are stained by ethidium bromide (upper panel) and commassie blue (lower panel) after gel electrophoresis. (C) The DLS analyses of hSerRS-tRNA complexes. The left vertical axis indicates the particle sizes of the species while the right vertical axis indicates the molecular weights of the major species in solution estimated by DLS. The horizontal axis indicates different molar ratios of protein to RNA.