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. 2015 Dec 1;9(12):e0004258. doi: 10.1371/journal.pntd.0004258

Fig 1. Transcriptional responses for mRNA encoding CYP following in vitro exposure of Opisthorchis felineus to xenobiotics.

Fig 1

Analysis by real-time PCR with normalization based on the expression stability value (M-value); normalization was undertaken using Ub and MrpL16 genes as endogenous internal controls (M < 1.2). Triplicate real-time PCRs were run for each sample. A. An adult worm; B. Newly excysted metacercariae (NEM). C. CYP mRNA level after in vitro treatment of O. felineus for 20 h with hemoglobin (DDPCR). CYP gene expression levels were quantified and values were simultaneously normalized to reference gene MrpL16 expression using QuantaLife (Bio-Rad, USA). The data are shown as the normalized ratio of CYP to MrpL16 ± S.D. Each run was made in duplex. Results of three independent experiments are presented.