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. 2015 Dec 1;9(12):e0004258. doi: 10.1371/journal.pntd.0004258

Fig 2. Representative chromatogram of chlorzoxazone metabolism in Opisthorchis felineus.

Fig 2

(a scanned copy of the original print version). A. extract of O. felineus incubation media without chlorzoxazone; B. extract of O. felineus incubation media containing 10 mM chlorzoxazone; C. extract of O. felineus incubation media containing 10 mM chlorzoxazone and 40 μМ ketoconazole; A peak corresponding to retention time of 6OH-CLZ in the 2c chromatogram is indicated with an arrow. D. extract c spiked with a standard of 6- hydroxychlorzoxazone; E structures of 6-OH-hydroxychlorzoxazone (1), chlorzoxazone (2), benzoxazole (is); F. Analysis of relative 6OH-CLZ amount. The area of the 6OH-CLZ peak in each chromatogram was measured. Data were expressed as a fold difference compared to the control. Data are presented as means ± S.D. Results are averaged from three independent experiments.