Table 1. Sequence of DNA oligonucleotides used in this study.
| Oligonucleotides | Sequence (5′→3′) |
|---|---|
| CF | GTGAGGAGCGATCTCAGCA |
| CR | GGAGCGGAGGGTAGTAATGA |
| BF | CCCTCTCAACCCTCTGTGTC |
| DR | TTCAAGGCCCTTTCTGCTTA |
| 7F | CACCTTGATGACTTCCTATCC |
| 9R | GAAATGGTGTTGGGGCTAAA |
| SD6 | TCTGAGTCACCTGGACAACC |
| SA2 | GCTCACAAATACCACTGAGAT |
| 6His-Fa | CCAGCGGATCCCCCTCAAAAATGCATCATCACCATCACCACATGTCGCGCAGTGGAACCGATC |
| 6His-Ra | GATCGGTTCCACTGCGCGACATGTGGTGATGGTGATGATGCATTTTTGAGGGGGATCCGCTGG |
| IVS8-Mut-Fb | CCCCTGCTGAGCGTGATGGTCAGTCTCCCAGATCTAGCCTCCATCATG |
| IVS8-Mut-Rb | CATGATGGAGGCTAGATCTGGGAGACTGACCATCACGCTCAGCAGGGG |
a
Underlined residues encode the hexa-histidyl tag.
b
Underlined residues represent the first 13 nucleotides of intron 8.