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. 2015 Dec 1;10(12):e0143897. doi: 10.1371/journal.pone.0143897

Fig 2. HS-5 stroma activates crucial signalling pathways in SET-2 cells.

Fig 2

SET-2 cells were cultured in vitro (no stroma), co-cultured in a stromal layer of HS-5 cells (+ HS-5) and with HS-5 conditioned media [+ CM (HS-5)] at the indicated time point the cells. Cells were lysed and the phosphorylation and total levels of STAT5, STAT3, JNK/SAPK, ERK1/2, Akt/PKB, GSK3α/β, S6 and p65/RELA were analyzed by immunoblot. Actin was used as loading control. The data is representative of two independent experiments.