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. 2015 Dec 1;10(12):e0143897. doi: 10.1371/journal.pone.0143897

Fig 3. Pharmacological inhibition of JNK and PI3K signalling pathways completely reverted HS-5 BM stroma mediated protection of SET-2 cells.

Fig 3

(A and B) SET-2 cells were cultured in vitro (no stroma) and co-cultured in a stromal layer of HS-5 cells (+ HS-5) for 72h in the presence of 2.0μM Vorinostat (Vor.), 500nM Ruxolitinib (Rux.), 5μM and 10μM SP600125 (JNK inhibitor) (in A) and 5μM and 10μM LY294002 (PI3K inhibitor) (in B). After 72h, the SET-2 cells were harvested, stained with CD45 (to distinguish between SET-2 and the stromal cell lines) and Annexin-V/PI or PI alone to determine cellular viability by Flow Cytometry analysis as described in “Materials and Methods”. The panels in (A and B) show the Viability Index graphs that normalize the viability values to those of the control condition (non-treated condition NT). Values indicate the mean ± standard deviation of the five experiments performed (* 0.05 >p; ** 0.01>p; *** 0.001>p).