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. 2015 Dec 1;10(12):e0143833. doi: 10.1371/journal.pone.0143833

Table 1. Nutritional content of the experimental diets (g/kg diet).

Nutritional content* normal chow LNAA diet high-protein diet
Carbohydrates 674 550 551
Fat 41 41 41
Dietary fibre 50 50 50
Protein 124 248 248
Amino acids**
LNAA L-Phenylalanine 6.0 6.1 (0.1) 12.2 (6.2)
L-Tyrosine 4.8 20.1 (15.3) 12.1 (7.3)
L-Valine 7.4 25.0 (17.6) 15.9 (8.5)
L-Isoleucine 5.9 22.9 (17.0) 12.4 (6.4)
L-Leucine 10.9 28.0 (17.1) 23.1 (12.2)
L-Methionine 3.0 19.7 (16.7) 6.9 (3.9)
L-Histidine 3.2 19.0 (15.8) 6.8 (3.5)
L-Threonine 5.3 5.7 (0.3) 11.3 (5.9)
non-LNAA L-Aspartic acid 9.3 9.7 (0.4) 19.4 (10.1)
L-Serine 7.7 7.9 (0.2) 15.8 (8.1)
L-Glutamic acid 28.2 29.9 (1.7) 59.6 (31.4)
Glycine 2.7 2.7 (0.0) 5.2 (2.5)
L-Alanine 3.9 4.2 (0.3) 8.2 (4.3)
L-Lysine 9.1 9.9 (0.8) 19.5 (10.4)
L-Arginine 4.2 4.7 (0.5) 9.5 (5.3)

Contents are not shown for L-Tryptophan, L-Proline, and L-cyst(e)ine, as these were not measured due to technical limitations. Differences with LNAA contents of normal chow are stated in brackets. LNAA, large neutral amino acid.

*Mineral and vitamin premixes were also included in accordance with the composition of the AIN93M diet [30].

**Dietary contents as measured in samples of prepared food pellets.