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. 2015 Dec 1;10(12):e0143802. doi: 10.1371/journal.pone.0143802

Fig 1. Fn14 expression in human tissue and cultured cells in response to TGF-β signaling.

Fig 1

(A) Fn14 expression in leukocytes from healthy donors and burn patients was detected by RT–qPCR. n = 3–5. (B) Fn14 expression in skin tissue from healthy donors (normal) and burn patients (scar) was detected by RT–qPCR. n = 3–5. (C) Fn14 mRNA expression was detected by RT–qPCR in human dermal fibroblasts (HDFs) in response to TGF-β signaling. (D) Fn14 protein expression in human dermal fibroblasts (HDFs) in response to TGF-β signaling was detected by western blotting. Human dermal fibroblasts (HDFs) were treated with TGF-β1, SB431542 or TGF-β1 plus SB431542. The protein expression data in the histogram was calculated using gray scale western blots of HDFs cultured in six-well plates. Total RNA was isolated from human tissues and cultured cells using TRIzol Reagent, and cDNA was synthesized for RT–qPCR. Data from at least three independent experiments are shown. Data are presented as the mean ± SD (standard deviation).