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. 2015 Dec 1;10(12):e0143802. doi: 10.1371/journal.pone.0143802

Fig 2. Fn14 overexpression in HDFs promotes fibroblast activation.

Fig 2

(A) Cultured HDFs were transfected with pcDNA3.1 (control) or pcDNA3.1-hFn14 (p-Fn14). (B) Fn14 mRNA expression in HDFs was detected by RT–qPCR after Fn14 overexpression. (C) Fn14 protein expression in HDFs was detected by western blotting after Fn14 overexpression. (D) COL1A1 mRNA expression was detected by RT–qPCR. (E) COL3A1 mRNA expression was detected by RT–qPCR. (F) COL1 protein expression was detected by western blotting. (G) αSMA protein expression was detected by western blotting. The protein expression data in the histogram were calculated using gray scale western blots. Data from at least three independent experiments are shown. Data are presented as the mean ± SD.