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. 2015 Dec 1;10(12):e0143802. doi: 10.1371/journal.pone.0143802

Fig 3. Fn14 knockdown via siRNA in HDFs inhibits fibroblast activation.

Fig 3

(A) Fn14 was knocked down in cultured HDFs with Fn14 siRNA, and an siRNA negative sequence was used as a control (negative control). (B) Fn14 mRNA expression was detected in Fn14 siRNA-transfected cells and control cells by RT-qPCR. (C) Fn14 protein expression in Fn14 siRNA-transfected cells and control cells was detected by western blotting. (D) COL1A1 mRNA expression in HDFs transfected with or without Fn14 siRNA was detected by RT-qPCR. (E) COL3A1 mRNA expression in HDFs transfected with or without Fn14 siRNA was detected by RT-qPCR. (F) αSMA protein expression in HDFs transfected with or without Fn14 siRNA was detected by western blotting. (G) COL1 protein expression in HDFs transfected with or without Fn14 siRNA was detected by western blotting. The protein expression data in the histogram were calculated using gray scale western blots. Data from at least three independent experiments are shown. Data are presented as the mean ± SD.