Fig 5. Detection of SMAD4 binding to the human Fn14 promoter in HDFs.
(A) The human Fn14 gene promoter was analyzed, and primers for chromatin immunoprecipitation (ChIP) were designed according to the sequences of potential binding sites. (B)-(I) ChIP-qPCR was performed with primer sets P-1 (B), P-2 (C), P-3 (D), P-4 (E), P-5 (F), P-6 (G), P-7 (H) and P-8 (I). Data from at least three independent experiments are shown. Data are presented as the mean ± SD.