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. 2015 Dec 1;11(12):e1005267. doi: 10.1371/journal.ppat.1005267

Fig 3. Expression monitoring of Hco-acr-26 and Hco-acr-27 in Haemonchus contortus.

Fig 3

(A) Transcription of Hco-acr-26 and Hco-acr-27 throughout the H. contortus lifecycle as revealed by RT-PCR experiments. ω: embryonated egg; L2: second stage larvae; L3: ensheated third stage larvae; XL3: in vitro exsheated third stage larvae, L4: fourth stage larvae; m: adult males; f: adult females. Integrity of cDNA preparations was verified by PCR using primers designed to amplify a 380 bp fragment of the H. contortus gapdh cDNA. (B) Relative mRNA expression levels of Hco-acr-26 and Hco-acr-27 in H. contortus L3 and adult males. Real-time RT-PCR experiments were performed in triplicate for third stage larvae (L3) and adult males corresponding to free-living and parasitic stage respectively. Each set of experiments was repeated twice using two independent cDNAs templates. The mRNA expression level for Hco-acr-26 was normalized to 1. The mRNA fold changes were calculated using three distinct reference genes encoding for gapdh, actin and β-tubulin. The data are presented as fold changes in mean ± SEM of mRNA expression. No difference was observed between relative mRNA expression of Hco-acr-26 and Hco-acr-27 in L3 and adult males (paired Student’s t-test).