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. 2015 Dec 1;11(12):e1005267. doi: 10.1371/journal.ppat.1005267

Fig 6. Identification of the minimal subunit combination and ancillary proteins required for the functional expression in Xenopus oocyte of AChR containing ACR-26 and ACR-27 from H. contortus and P. equorum.

Fig 6

For both parasitic nematode species, the robust expression of functional AChRs required the co-injection of cRNAs corresponding to ACR-26 and ACR-27 AChR subunits in combination with the three ancillary factors RIC-3.1; UNC-50 and UNC-74. Currents were measured at the plateau. (A) Average plateau values for co-injection of all cRNAs were 161.4±18.67 nA (n = 41) for H. contortus receptor. (B) Average plateau values for co-injection of all cRNAs were 246.8±33.39 nA (n = 37) for P. equorum receptor. Results are shown in mean ± SEM.