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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Nat Methods. 2015 Sep 7;12(11):1051–1054. doi: 10.1038/nmeth.3580

Figure 1.

Figure 1

Activation and cutting of endogenous genes in HEK293T cells. (a–c) RNA expression and mutagenesis analysis of the genes ACTC1 (a), MIAT (b) and TTN (c). Each sample was transfected with the indicated Cas9 construct and gRNA of a particular length. Points represent data from two individual transfections. (d) Multiplexed activation and cutting of ACTC1, MIAT and TTN. The constructs were transfected with 20-nt ACTC1 gRNA and 14-nt MIAT and TTN gRNAs simultaneously. Points represent data from two individual transfections.