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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Exp Hematol. 2015 Sep 11;43(12):1031–1046.e12. doi: 10.1016/j.exphem.2015.08.013

Figure 5. FANCA shuttles to the pericentriolar material during mitosis.

Figure 5

(A) HeLa cells were immunostained with antibodies against endogenous FANCA (red) and centrin (green), imaged with deconvolution microscopy (Applied Precision personalDx) and deconvolved with Softworx imaging suite (10 iterations, ratio: conservative). Fluorescence intensity profiles demonstrate that FANCA colocalizes with centrin in interphase and migrates away from centrioles at metaphase. Scale bars: 1.5 µm (left) and 300 nm (right) (B) Representative super-resolution image of human fibroblast stably expressing GFP-FANCA and stained with antibody against the pericentriolar material marker (pericentrin). Inserted 3D rendering of the centrosome shows colocalization of GFP-FANCA and pericentrin. Scale bars: 2 µm. The yellow region of interest is magnified (C) to show FANCA fibers embedded within the PCM (centrosome cross-section) and extending towards the spindle (centrosome outer layer section). Fluorescence intensity profiles (right) of GFP-FANCA/pericentrin signal at PCM and spindle are shown on the right. Scale bar: 500 nm. SR-SIM images were acquired on Zeiss ELYRA PS.1 super-resolution microscopy system and exported using Imaris imaging suite.