Skip to main content
. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Nat Methods. 2015 Oct 26;12(12):1143–1149. doi: 10.1038/nmeth.3630

Figure 3. Genome-wide binding activity of dCas9 repressors targeted to the HS2 enhancer.

Figure 3

(a) ChIP-seq tracks demonstrate highly specific binding of FLAG-tagged dCas9 and dCas9-KRAB to the HS2 enhancer (shaded region) of the globin locus (chr11: 5244651 – 5314450), compared to dCas9-KRAB without sgRNA. An ENCODE K562 DNase I hypersensitivity DNase-seq track is included to highlight the globin LCR22. (b,c) Differential analyses of global binding activity include comparisons of dCas9-KRAB targeted by (b) Cr4 and (c) Cr10 versus dCas9-KRAB without sgRNA. Points labeled in red indicate FDR < 0.05 by differential DESeq analysis (n = 3 biological replicates).