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. Author manuscript; available in PMC: 2016 Sep 10.
Published in final edited form as: Oncogene. 2015 Jun 1;35(10):1250–1260. doi: 10.1038/onc.2015.179

Figure 5.

Figure 5

Effects of oncogene expression and SREBP depletion on cell growth and proliferation in MCF10a cells. (a,c) Proliferation of PIK3CAH1047R- and K-RasG12V-expressing MCF10a cells compared to vector-expressing cells cultured in full growth medium (a) or in low serum conditions (c) starting 24 h post-knockdown (t = 0 h). For all proliferation graphs, time points are shown as mean ± s.e.m., n=3. * P-value < 0.05 compared to control cells at the final time point. (b,d) Effect of SREBP depletion on proliferation of oncogene-expressing MCF10a cells. Cells from a and c cultured in full serum (b) or low serum (d) were counted every 24 h following the siRNA-mediated knockdown of SREBP1, SREBP2, or both. (e,f) Oncogene- and SREBP-dependent effects on cell growth. The diameters of the cells treated as in c (e) and d (f) were measured at 48 h, following trypsinization. Color-coded P-values, compared to cells with control siRNAs, correspond to the color-coding in the legend (>1000 cells measured for each).